Skip to main content
. 2012 Nov 12;7(11):e49252. doi: 10.1371/journal.pone.0049252

Figure 3. GroEL responding CD4+ T cells express IFNγ, IL-10, TNFα and T-bet.

Figure 3

PBMCs cultured from 24 h to 96 h with rAaGroEL (20 µg/ml). RPMI and PMA/I or PMA were used as negative and positive controls respectively. At indicated time points CD4 cell surface staining was performed. Then, cytokine antibodies (IFNγ, IL10, TNFα and T-bet) were added. Cells were acquired and analyzed. For each molecule analysis, cells were gated for CD4+ T cells. (A), (B), (C), (D) shows time kinetics of IFNγ, IL-10, TNFα and T-bet expression among CD4+ T cells with controls. Data are representative of 3 experiments. Error bars represent standard deviation and *represents p<0.05.