Figure 2
|
A (TNF-α, 30 min He, 4 h LPS)
|
B (TNF-α, 30 min He, 24 h LPS)
|
C (TNF-α, 60 min He, 4 h LPS)
|
D (TNF-α, 60 min He, 24 h LPS)
|
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
T0
|
0.68
|
12
|
12
|
12
|
0.42
|
12
|
12
|
12
|
0.84
|
6
|
6
|
6
|
0.53
|
6
|
6
|
6
|
T1
|
0.91
|
12
|
12
|
12
|
0.73
|
12
|
12
|
12
|
0.44
|
6
|
6
|
6
|
0.35
|
6
|
6
|
6
|
T2
|
0.73
|
12
|
12
|
12
|
0.52
|
12
|
12
|
12
|
0.06
|
5
|
6
|
5
|
0.50
|
6
|
6
|
6
|
T3
|
0.38
|
12
|
12
|
12
|
0.79
|
12
|
12
|
12
|
0.81
|
5
|
5
|
6
|
0.72
|
6
|
6
|
6
|
T4
|
0.27
|
12
|
12
|
12
|
0.73
|
12
|
12
|
12
|
0.44
|
5
|
5
|
6
|
0.53
|
6
|
6
|
6
|
T5
|
0.52
|
12
|
12
|
12
|
0.68
|
12
|
12
|
12
|
0.56
|
6
|
6
|
6
|
0.70
|
6
|
6
|
6
|
|
E (IL-6, 30 min He, 4 h LPS)
|
F (IL-6, 30 min He, 24 h LPS)
|
G (IL-6, 60 min He, 4 h LPS)
|
H (IL-6, 60 min He, 24 h LPS)
|
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
T0
|
0.91
|
12
|
12
|
12
|
0.65
|
10
|
10
|
11
|
1.00
|
6
|
6
|
6
|
0.09
|
6
|
6
|
6
|
T1
|
0.83
|
11
|
11
|
12
|
0.08
|
11
|
11
|
12
|
0.16
|
6
|
6
|
6
|
0.44
|
6
|
6
|
6
|
T2
|
0.42
|
12
|
12
|
12
|
0.62
|
10
|
10
|
12
|
0.44
|
6
|
6
|
6
|
0.84
|
6
|
6
|
6
|
T3
|
0.91
|
12
|
12
|
12
|
0.11
|
11
|
11
|
11
|
1.00
|
4
|
4
|
5
|
1.00
|
6
|
6
|
6
|
T4
|
0.76
|
11
|
12
|
11
|
0.23
|
10
|
10
|
12
|
1.00
|
5
|
6
|
5
|
0.44
|
6
|
6
|
6
|
T5
|
1.00
|
12
|
12
|
12
|
0.77
|
11
|
11
|
11
|
0.69
|
5
|
6
|
5
|
0.69
|
6
|
6
|
6
|
Figure 3
|
A (IL-1β, 30 min He, 4 h LPS)
|
B (IL-1β, 30 min He, 24 h LPS)
|
C (IL-1β, 60 min He, 4 h LPS)
|
D (IL-1β, 60 min He, 24 h LPS)
|
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
T0
|
0.30
|
12
|
12
|
12
|
0.28
|
12
|
12
|
12
|
1.00
|
6
|
6
|
6
|
1.00
|
5
|
6
|
5
|
T1
|
0.42
|
12
|
12
|
12
|
0.42
|
12
|
12
|
12
|
0.56
|
6
|
6
|
6
|
0.44
|
6
|
6
|
6
|
T2
|
0.34
|
12
|
12
|
12
|
0.30
|
12
|
12
|
12
|
0.19
|
6
|
6
|
6
|
0.31
|
6
|
6
|
6
|
T3
|
0.13
|
12
|
12
|
12
|
0.97
|
12
|
12
|
12
|
0.44
|
5
|
5
|
5
|
0.44
|
6
|
6
|
6
|
T4
|
0.70
|
11
|
12
|
11
|
0.97
|
12
|
12
|
12
|
1.00
|
6
|
6
|
6
|
1.00
|
6
|
6
|
6
|
T5
|
1.00
|
12
|
12
|
12
|
0.34
|
12
|
12
|
12
|
0.69
|
6
|
6
|
6
|
0.69
|
6
|
6
|
6
|
|
E (IL-8, 30 min He, 4 h LPS)
|
F (IL-8, 30 min He, 24 h LPS)
|
G (IL-8, 60 min He, 4 h LPS)
|
H (IL-8, 60 min He, 24 h LPS)
|
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
T0
|
0.34
|
12
|
12
|
12
|
0.46
|
10
|
10
|
10
|
0.22
|
6
|
6
|
6
|
0.22
|
6
|
6
|
6
|
T1
|
0.34
|
12
|
12
|
12
|
0.47
|
9
|
10
|
9
|
0.31
|
6
|
6
|
6
|
0.84
|
6
|
6
|
6
|
T2
|
0.20
|
12
|
12
|
12
|
0.31
|
10
|
10
|
10
|
0.56
|
6
|
6
|
6
|
0.44
|
6
|
6
|
6
|
T3
|
0.51
|
12
|
12
|
12
|
0.43
|
11
|
11
|
11
|
0.44
|
6
|
6
|
6
|
0.16
|
6
|
6
|
6
|
T4
|
0.91
|
11
|
12
|
11
|
0.50
|
10
|
11
|
10
|
0.84
|
6
|
6
|
6
|
0.44
|
6
|
6
|
6
|
T5
|
0.97
|
12
|
12
|
12
|
0.13
|
10
|
10
|
11
|
1.00
|
6
|
6
|
6
|
0.84
|
6
|
6
|
6
|
Figure 4
|
A (TNF-α, 30 min He, 4 h LTA)
|
B (TNF-α, 30 min He, 24 h LTA)
|
C (IL-6, 30 min He, 4 h LTA)
|
D (IL-6, 30 min He, 24 h LTA)
|
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
T0
|
0.90
|
12
|
12
|
12
|
0.68
|
12
|
12
|
12
|
0.24
|
11
|
12
|
11
|
0.55
|
11
|
11
|
12
|
T1
|
0.68
|
12
|
12
|
12
|
0.76
|
12
|
12
|
12
|
0.89
|
11
|
12
|
11
|
0.02*
|
11
|
11
|
12
|
T2
|
0.73
|
12
|
12
|
12
|
0.79
|
12
|
12
|
12
|
0.05
|
11
|
11
|
11
|
0.64
|
11
|
11
|
12
|
T3
|
0.30
|
12
|
12
|
12
|
0.94
|
12
|
12
|
12
|
0.24
|
11
|
12
|
11
|
0.24
|
11
|
11
|
12
|
T4
|
0.20
|
11
|
12
|
11
|
0.38
|
11
|
12
|
11
|
0.07
|
11
|
11
|
12
|
0.05
|
11
|
11
|
12
|
T5
|
0.97
|
12
|
12
|
12
|
0.91
|
12
|
12
|
12
|
0.41
|
11
|
12
|
12
|
0.32
|
11
|
11
|
12
|
|
E (IL-1β, 30 min He, 4 h LTA)
|
F (IL-1β, 30 min He, 24 h LTA)
|
G (IL-8, 30 min He, 4 h LTA)
|
H (IL-8, 30 min He, 24 h LTA)
|
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
T0
|
0.89
|
11
|
11
|
12
|
1.00
|
11
|
11
|
12
|
0.34
|
12
|
12
|
12
|
0.19
|
8
|
8
|
10
|
T1
|
0.62
|
12
|
12
|
12
|
0.44
|
11
|
11
|
12
|
0.34
|
12
|
12
|
12
|
0.63
|
8
|
9
|
8
|
T2
|
0.34
|
12
|
12
|
12
|
0.31
|
11
|
11
|
12
|
0.20
|
12
|
12
|
12
|
0.74
|
10
|
10
|
11
|
T3
|
0.97
|
12
|
12
|
12
|
0.44
|
11
|
11
|
12
|
0.51
|
12
|
12
|
12
|
0.81
|
9
|
9
|
10
|
T4
|
0.38
|
12
|
12
|
12
|
1.00
|
11
|
11
|
12
|
0.91
|
11
|
12
|
11
|
0.07
|
11
|
11
|
11
|
T5
|
1.00
|
11
|
11
|
11
|
0.69
|
11
|
11
|
12
|
0.97
|
12
|
12
|
12
|
0.20
|
11
|
11
|
11
|
Figure 5
|
A (IL-2, 30 min He, 4 h TCS)
|
B (IL-2, 30 min He, 24 h TCS)
|
C (IFN-Υ, 30 min He, 4 h TCS)
|
D (IFN-Υ, 30 min He, 24 h TCS)
|
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
p
|
n
|
n He
|
n Air
|
T0
|
0.76
|
11
|
11
|
11
|
0.83
|
11
|
11
|
11
|
0.92
|
11
|
11
|
11
|
0.16
|
11
|
11
|
11
|
T1
|
0.83
|
11
|
11
|
11
|
0.97
|
11
|
11
|
11
|
1.00
|
11
|
11
|
11
|
0.16
|
10
|
11
|
10
|
T2
|
0.58
|
11
|
11
|
11
|
0.46
|
11
|
11
|
11
|
0.01*
|
11
|
11
|
11
|
0.91
|
10
|
10
|
10
|
T3
|
0.76
|
11
|
11
|
11
|
0.85
|
11
|
11
|
11
|
0.38
|
11
|
11
|
11
|
0.16
|
10
|
12
|
10
|
T4
|
0.28
|
11
|
11
|
11
|
0.10
|
11
|
11
|
11
|
0.13
|
11
|
11
|
11
|
0.85
|
10
|
12
|
10
|
T5 |
0.28 |
11 |
11 |
11 |
0.58 |
11 |
11 |
11 |
1.00 |
11 |
11 |
11 |
0.01* |
10 |
11 |
10 |