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. 2012 Nov 12;7(11):e49007. doi: 10.1371/journal.pone.0049007

Figure 3. Spatial requirement of Ser activity at the ectopic FB during wing development.

Figure 3

Clones are labelled by the absence of the GFP or RFP marker. (A, C, E, G) Ser clones abutting the posterior side of the A/P boundary do not affect the expression of Wg in early (A, G) as well as in late (C, E) stages. The arrowheads highlight the clone abutting the posterior side. (E) A disc in the late stages of third larval instar bearing a large Ser clone abutting the posterior boundary. (E’’) Magnification of the region highlighted in (E’) by the arrowhead. It reveals that the symmetric late phase of Wg expression is normally established indicated by the expression of Wg in the mutant territories in late stages (arrow in E’’). (B, D, F, H) Ser clones abutting the anterior side had variable effects. Arrowheads highlight the clones. A fraction of discs showed a strong shortening of the ectopic expression domain of Wg in the mutant area (B, D). In other cases the expression appears to be unaffected (F, H). (I) In these cases the expression in late stages remains restricted to ABCs. Thus, the symmetric phase is not established in the mutant region.