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. 2012 Nov 12;7(11):e49007. doi: 10.1371/journal.pone.0049007

Figure 6. Clonal analysis of the null allele Su(H)d47 with the MARCM system.

Figure 6

Clones are labelled positively with the GFP marker. (A) Expression of Ap in Su(H)d47 null mutant wing imaginal discs. The boundary between the Ap expressing and non-expressing cells (arrow) is smooth, indicating that the D/V compartment boundary has formed in the absence of Su(H) function. (B–P) Clones of Su(H) abutting the dorsal and/or ventral side of the D/V boundary. The dorsal cells are additionally labelled with Ser-lacZ. (B–D) A clone that crosses the D/V boundary causes loss of expression of Wg along the D/V boundary (arrow). (E-I) A Clone abutting the dorsal side of the D/V boundary causes a thinning, but no loss of the Wg expression domain. (J–N) In contrast, clones abutting the ventral side of the D/V boundary completely abolish expression of Wg and the vgBE (highlighted by the arrows). (O–S) A clone abutting the ventral side of the D/V boundary in an early third instar disc. Wg is interrupted in the area of the clone (arrow).