Table 1. Primers used for quantification of Total bacteria, Bacteroides-Prevotella, Bifidobacteria and Lactobacillus.¹.

Target group	Primer Name	Sequence (5’-3’)	Coverage2	Specificity3	Reference
Total bacteria	Uni331F	TCCTACGGGAGGCAGCAGT	ND4	ND4	Jernberg et al. 2007
	Uni797R	GGACTACCAGGGTATCTATCCTGTT
Bacteroides-Prevotella	AllBac296F	GAG AGG AAG GTC CCCCAC	73.3	86.0	Cani et al. 2008
	AllBac2112R	CGCTACTTGGCTGGTTCAG
Bifidobacterium	Bif164F	GGGTGGTAATGCCGGATG	24.6	99.3	Kok et al. 1996
	Bif662R	CCACCGTTACACCGGGAA
Lactobacillus	LacF	AGCAGTAGGGAATCTTCCA	68	89.4	Rinttila et al. 2004
	LacR	CACCGCTACACATGGAG

¹Coverage was determined by the probe match function on the Ribosomal Database Project. Accessed 4/26/2012. ²Percentage of the database’s total number of organism sequences from the target groups that the primers match. ³Percentage of amplicons matched across the entire database that correspond to organisms of the target group. ⁴ND = not determined.