Table 2.
Nucleotide sequence variations in the genes of MEV/LN-10 and amino acid variations in the NS1 and VP2 proteins of the MEV/LN-10 isolate
| nt position | 28 | 288 | 741 | 743 | 1053 | 1107 | 1224 | 1251 | 1362 | 1401 | 1419 | 1576 | 1619 | 1633 | 1633 | 1686 | 1720 | 1785 | 1846 | 1887 | 1926 | 1959 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| aa position of NS1 |
10 |
* |
247 |
248 |
351 |
* |
* |
* |
* |
* |
* |
* |
540 |
545 |
* |
* |
574 |
595 |
616 |
629 |
* |
* |
| MEVB |
I |
c |
H |
T |
N |
a |
t |
a |
t |
c |
g |
c |
V |
Q |
c |
c |
V |
H |
N |
H |
a |
t |
| MEV/LN-10 |
V |
t |
Q |
I |
N |
a |
c |
g |
c |
t |
a |
t |
A |
E |
g |
t |
I |
H |
D |
Q |
g |
c |
| CPV-447 |
V |
t |
Q |
I |
K |
g |
t |
g |
t |
t |
a |
t |
V |
E |
g |
t |
I |
Q |
D |
Q |
g |
c |
| nt position |
2076 |
2110 |
2355 |
2569 |
2586 |
2610 |
2640 |
2661 |
2753 |
2760 |
2773 |
2793 |
2816 |
2822 |
3051 |
3208 |
3213 |
3214 |
3403 |
3413 |
3427 |
3481 |
| aa position of VP2 |
* |
* |
* |
* |
* |
* |
* |
* |
80 |
* |
87 |
93 |
101 |
103 |
* |
232 |
* |
234 |
297 |
300 |
305 |
323 |
| MEVB |
g |
c |
a |
c |
a |
t |
a |
g |
K |
a |
M |
K |
I |
V |
t |
I |
t |
H |
S |
V |
D |
D |
| MEV/LN-10 |
a |
t |
g |
a |
g |
c |
g |
a |
K |
a |
M |
K |
T |
V |
t |
V |
t |
Y |
S |
V |
D |
D |
| CPV-447 |
g |
t |
g |
a |
g |
t |
g |
g |
R |
g |
L |
N |
T |
A |
c |
I |
c |
H |
A |
G |
Y |
N |
| nt position |
3552 |
3681 |
3746 |
3790 |
3918 |
4071 |
4137 |
4205 |
4217 |
|
|
|
|
|
|
|
|
|
|
|
|
|
| aa position of VP2 |
* |
* |
411 |
426 |
* |
* |
* |
564 |
568 |
|
|
|
|
|
|
|
|
|
|
|
|
|
| MEVB |
a |
t |
A |
N |
a |
g |
a |
N |
A |
|
|
|
|
|
|
|
|
|
|
|
|
|
| MEV/LN-10 |
a |
t |
E |
N |
a |
a |
a |
N |
A |
|
|
|
|
|
|
|
|
|
|
|
|
|
| CPV-447 | g | c | E | D | g | a | g | S | G |
a) aa: amino acid, nt: nucleotide.
b) An asterisk (*) indicates a synonymous substitution of the coding region.