Figure 2. Analysis of centrosomal localization of HDACs. To test whether HDACs localize to centrosomes, U2OS cells were transfected with each HDAC tagged with a FLAG-epitope, and co-immunostained with anti-γ-tubulin and anti-FLAG antibodies, followed by DAPI-staining for DNA. HDAC2, 3, 7, 8, 9 and SIRT3, 4, 5, 6, 7 were found to be negative for centrosome localization, and the images of the FLAG-HDAC2-transfected cells are shown as an example for the centrosome localization-negative staining. Arrows point to centrosomes. The insets show the magnified images of the centrosome areas. Scale bar: 10 μm.