Figure 10. P-ΔNp63α/SREBF1 and non-p-ΔNp63α/E2F1 protein complexes formed in SCC cells upon cisplatin exposure. (A) Immunoblotting analysis of SCC-11 and SCC-11M cells exposed to control medium (Con) and 10 μg/ml cisplatin (CIS). Nuclear lysates were probed for expression of specific transcription factors with indicated antibodies. Levels of TBP served as loading controls. (B) Immunoprecipitation (IP) with anti-p-ΔNp63α antibody followed by immunoblotting with anti-SREBF1 and NF-Y antibodies. (C) IP with anti-ΔNp63 antibody followed by immunoblotting with anti-E2F1 and anti-p53 antibodies.