Table 3.
Antigen | Manufacturer | Mono-/polyclonal | Staining | Positive control | Blocking | Concentration |
---|---|---|---|---|---|---|
phospho-Smad1 (Ser463/465) |
Cell signaling |
polyclonal rabbit IgG |
nucleus |
colon |
no |
1:200 |
/Smad5 (Ser463/465) | ||||||
/Smad8 (Ser426/428) | ||||||
phospho-Smad2 (Ser465/467) |
Cell signaling |
monoclonal rabbit IgG1 |
nucleus |
kidney |
10% normal goat serum, 30’ |
1:50 |
endoglin / CD105 | R&D systems | polyclonal goat IgG | cytoplasmic | tonsil | no | 1:800 |