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. 2012 Oct 22;12:488. doi: 10.1186/1471-2407-12-488

Table 3.

Antibodies used for immunohistochemical analysis

Antigen Manufacturer Mono-/polyclonal Staining Positive control Blocking Concentration
phospho-Smad1 (Ser463/465)
Cell signaling
polyclonal rabbit IgG
nucleus
colon
no
1:200
/Smad5 (Ser463/465)
/Smad8 (Ser426/428)
phospho-Smad2 (Ser465/467)
Cell signaling
monoclonal rabbit IgG1
nucleus
kidney
10% normal goat serum, 30’
1:50
endoglin / CD105 R&D systems polyclonal goat IgG cytoplasmic tonsil no 1:800