Table 1. List of primers used for real-time PCR.
Target | Accessionnumber | Forward primer5' to 3' | Reverse primer5' to 3' | Ampliconsize (bp) |
FSHR | NM_174061.1 | GCCAGCCTCACCTACCCCAGC | AATTGGATGAAGGTCAGAGGTTTGCC | 75 |
LHR | NM_174381.1 | ATTGCCTCAGTCGATGCCCAGACC | AAAAAGCCAGCCGCGCTGC | 92 |
IGF1R | XM_606794.3 | ACCTCCCAGCCTAAGCAAAATGATCC | TCTTCGGCCACCATGCAGTTCC | 123 |
STAR | NM_174189 | TTTTTTCCTGGGTCCTGACAGCGTC | ACAACCTGATCCTTGGGTTCTGCACC | 103 |
CYP11A1 | NM_176644 | CAGTGTCCCTCTGCTCAACGTCC | TTATTGAAAATTGTGTCCCATGCGG | 99 |
HSD3B1 | NM_174343.2 | GCCACCTAGTGACTCTTTCCAACAGCG | TGGTTTTCTGCTTGGCTTCCTCCC | 111 |
CYP17A1 | NM_174304 | GACAAAGGCACAGACGTTGTGGTCA | TGATCTGCAAGACGAGACTGGCATG | 301 |
CYP19A1 | NM_174305.1 | CGCCACTGAGTTGATTTTTGCTGAGA | TAAGGCTTTGCGCATGACCAGGTC | 301 |
HSD17B1 | NM_001102365 | CGCATATTGGTGACCGGGAGCATA | AATCGCCAGACTCTCGCACAAACC | 108 |
ACTB | NM_173979.3 | ATCACCATCGGCAATGAGCGGTTC | CGGATGTCGACGTCACACTTCATGA | 128 |