Figure 7. Normal CNC in adult Dicer1^Atoh7 mice. A,B,

Nissl stained CNC sections of adult (P42, 3 animals) wild type (A) or Dicer1^Atoh7 mice (P42, 3 animals) (B) at anterior (A1,B1), middle (A2,B2) or posterior (A3,B3) levels. No difference in shape or size was observed between wild type and Dicer1^Atoh7 mice. C,D, Nissl stained sections revealed normal formation of the SOC in Dicer1^Atoh7 mice. E, Genotyping PCR of DNA extracted from the CNC of indicated mouse lines. In homozygous Dicer1^Atoh7 mice, two alleles were present: 429 bp (recombined allele) and 767 bp (floxed allele), whereas in the CNC of wild type mice, a 560 bp fragment was amplified corresponding to the wild type allele. F, RT-PCR analysis of mRNA extracted from the CNC of a wild type or Dicer1^Atoh7 mouse. In wild type, only the full length sequence (1,500 bp) was amplified, whereas in Dicer1^Atoh7 mice, also the truncated sequence (360 bp) was amplified. Data are representative examples of 2 to 3 biologically independent experiments. These data confirm Cre-mediated recombination in the AVCN. fl, floxed allele; rec, allele after Cre-mediated recombination; wt, wild type allele.