Table 2.
Characteristics of microsatellite markers analyzed
| Name (locus) | Primer sequence (5’ to 3’) | Unit of repeats | PCR-Tmc | Dye | Size (bp) |
|---|---|---|---|---|---|
|
TP53-Alu |
F…GCA CTT TCC TCA ACT CTA CA |
5 |
55°C |
FAM |
382-417 |
| |
R…AAC AGC TCC TTT AAT GGC AG |
|
|
|
|
|
Mfd41 (D17S261) |
F…CAG GTT CTG TCA TAG GAC TA |
2 |
55°C |
NED |
153-172 |
| |
R…TTC TGG AAA CCT ACT CCT GA |
|
|
|
|
|
Mfd28 (D10S89) |
F…AAC ACT AGT GAC ATT ATT TTC |
2 |
55°C |
FAM |
139-156 |
| |
R…AGC TAG GCC TGA AGG CTT CT |
|
|
|
|
|
Bat-40 |
F…ATT AAC TTC CTA CAC CAC AAC |
1 |
55°C |
VIC |
116-132 |
| |
R…GTA GAG CAA GAC CAC CTT G |
|
|
|
|
|
Bat-26 |
F…TGA CTA CTT TTG ACT TCA GCC |
1 |
55°C |
FAM |
112-127 |
| R…AAC CAT TCA ACA TTT TTA ACC C |