Effect of 2,4-D treatment on d 0 on
SAMS expression in unpollinated pea ovaries. A, Ten
micrograms of total RNA extracted from 2,4-D-treated pea ovaries was
hybridized to SAMS gene-specific RNA probes, and RPA was
performed. d3 to d5, Days with respect to anthesis. B, Quantification
of the SAMS mRNAs at the stages shown in A.
SAMS transcript levels were quantified using a
radioanalytical imaging system. Light gray bars, SAMS1; dark gray bars,
SAMS2. The data were corrected for the number of uracil residues within
each probe.