Figure 3. Effects of growth factor treatment on the development of cultured tri-pronuclear zygotes.

Human tri-pronuclear zygotes were cultured individually in micro-drops containing serum-free media with or without key growth factors for up to 144 h. Morphological changes were monitored for blastocyst formation at early, expanded, and hatched stages. Numbers of embryos developed to specific stages vs. total number of embryos studied are listed on top of each column. *, P<0.05 vs. control. GFs: growth factors.