Table 1. Development of SCNT embryos cultured in media with or without growth factor supplementation.

	Without growth factors	With growth factors
Vitrified oocytes	34	29
Oocytes surviving the thawing	31	25
Oocytes used for enucleation	29	24
No. of successful enucleation	21	22
No. of successful fibroblast injection	14	15
No. of activated oocytesNo. of cleaved embryos	65	128
Embryos with ≥4-cells on day 3	1	6*
Embryos with >8-cells	0	1(16-cell)

Failed-to-be-fertilized oocytes were vitrified and then thawed before evaluation for morphology and SCNT using serum-starved fibroblasts arrested at the G₀/G₁ stage. After SCNT, reconstituted oocytes were activated using using 5 µM calcium ionophone for 10 min,followed by incubation for 4 h with 2.5 mM 6-dimethylaminopurine, and cultured in media with or without growth factors. Numbers of oocytes/embryos at each experimental stage are shown. *P = 0.187.