Table 2. UV Melting temperature (°C) Value of PNA: RNA and PNA: DNA Duplexes^*.

No.	PNA	PNA:RNA	PNA:DNA	ΔTm(RNA-DNA)
1	PNA 73	73.9	65	8.9
2	PNA 98	83.9	75.2	8.7
3	PNA 102	82.8	70.4	12.5
4	PNA 110	77.7	64.8	12.9
5	PNA 237	83.5	64	19.5
6	PNA 251	84.8	77.3	7.5
7	PNA 271	80.9	64	16.9
8	PNA 332	81.4	72	9.4

^*All values are average of at least three experiment. Buffer- 10 mM sodium phosphate buffer, pH 7 containing NaCl (100 mM) and EDTA (0.1 mM).