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. 2012 Nov 12;7(11):e49292. doi: 10.1371/journal.pone.0049292

Figure 5. Transcript (A, B) and protein analysis (C) of Pa_2_4660 (PaSod2).

Figure 5

The results of the SuperSAGE analysis (A) are shown as tags per million (tpm) for wild type (WT) and grisea (gr). The results of the qRT-PCR (B) are relative expression levels of the gene of the wild type (WT, n = 5) and of grisea (gr, n = 6) grown in standard medium, and wild type (WT+Cu, n = 3) and grisea (gr+Cu, n = 3) grown on copper supplemented medium, normalized to the expression level of gene coding for mitochondrial PORIN. The error bars represent the standard deviation. For Western Blot (α-PaSOD2) and in-gel SOD activity assay (C) 100 µg of total protein extracts of three independent wild type (WT) and grisea (gr) isolates were used. As loading control the α-PaPRE3 antibody was used.