Fig. 3. Anti-PECAM conjugated liposomes bind specifically to endothelial cell targets in vitro.

(a) Binding of Ab-MJ33/IL fluorescently labeled with the inclusion of CF-DSPE compared to (b) IgG-ILs. (c) Binding of anti-PECAM (Ab62) and IgG liposomes to cultured HUVECs quantified by radiotracing ¹²⁵I labeled IgG dosed with unlabeled mAbs at 10% by mass. Expressed data was calculated by comparing radioactivity measures per well of lysed cells post incubation normalized to cell number relative to the activity of known activity and concentration of the liposome aliquot added. n=4, Error bars represent standard deviation.