Fig. 6. Inhibition of pro-inflammatory endothelial activation by targeted MJ33 liposomes in vitro and in vivo.
(a) Quantification of VCAM expression normalized to actin from cells treated +/- anti-PECAM (Ab62) ILs challenged by 10 ng/ml TNF for 4 hr. Resulting cell lysates were measured for VCAM by Western blot. Error bars represent standard deviation. **p < 0.005. (b) Quantification of VCAM expression normalized to actin in mice lung homogenates +/- anti-PECAM (Mec13) ILs challenged by intratracheally administered LPS (I mg/kg) 24 hr prior to lung harvest. Resulting lung homogenates were measured for VCAM by Western blot. Error bars represent standard deviation, n=3 for targeted and untargeted ILs. **p<0.001