Figure 2.

For a dual-luciferase assay, control calvarial osteoblasts were transfected with (1) pGL3-P3-Luc reporter vector and (2) one of five ALX4 isoforms in a pCMV6 vector with minimal promoter. Transfection of pGL3-P3-Luc (P3 binding element) alone resulted in negligible luciferase production. Six independent replicates were tested for each construct and the results are expressed as the mean ± SEM. Renilla vectors were used as internal transfection controls for all experiments.