Fig. 2.

hCtr1 mRNA levels in cDDP^R cells and their parental cells treated with different Cu-lowering agents. (A – C) RPA of endo-hCtr1 mRNA expression levels in three pairs of cells, i.e., 2008 (cDDP^S)/2008-cp (cDDP^R), A172 (cDDP^s)/A172-CP (cDDP^R), and SCLC (cDDP^S)/SR2 (cDDP^R), treated with different concentrations of TM (upper row), trientine (middle), or D-pen (lower) as indicated for 16 h. 18S RNA was used as an internal control. (D) Measurements of ⁶⁴Cu uptake in three pairs of cDDP^R/cDDP^S cell lines with or without Cu-lowering agent added. (E) cDDP uptake measurements. Cells were treated with TM (5 µM), trientine or D-Pen (100 µM each) for 16 h. Greater sensitization of cDDP^R cells to cDDP (F), carboplatin (G), but not oxaliplatin (H) than their parental cells, after treatment with Cu-lowering agents was shown *, p ≤ 0.05, Student’s t-test.