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. Author manuscript; available in PMC: 2013 Dec 1.

Published in final edited form as: J Surg Res. 2012 Jun 1;178(2):632–639. doi: 10.1016/j.jss.2012.05.044

Panel 2A shows that surgical manipulation of the rat gastrointestinal tract initiated a temporal progressive increase in the qPCR expression of MMP-9 mRNA within the small intestinal (left panel) and colonic (right panel) isolated muscularis externa. MMP-9 mRNA was significantly increased at all time points measured between 3 and 24 hours after surgical manipulation compared to control (N=6, p ≤ 0.05, one-way ANOVA with posthoc Bonferoni analysis). Panel 2B is a representative electrophoretic zymography gel experiment in which a significant increase in MMP-9 enzymatic activity could be demonstrated in the peritoneal fluid harvested 3, 6 and 24 hours after surgical manipulation.

Panel 2A shows that surgical manipulation of the rat gastrointestinal tract initiated a temporal progressive increase in the qPCR expression of MMP-9 mRNA within the small intestinal (left panel) and colonic (right panel) isolated muscularis externa. MMP-9 mRNA was significantly increased at all time points measured between 3 and 24 hours after surgical manipulation compared to control (N=6, p ≤ 0.05, one-way ANOVA with posthoc Bonferoni analysis). Panel 2B is a representative electrophoretic zymography gel experiment in which a significant increase in MMP-9 enzymatic activity could be demonstrated in the peritoneal fluid harvested 3, 6 and 24 hours after surgical manipulation.