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Microplate ELISA measurement of the tissue inhibitor of metalloproteinase-1 (TIMP-1) protein levels was performed on peritoneal fluid and serum harvested from controls and surgically manipulated rats 3, 6 and 24 hours after resuscitation. TIMP-1 protein levels were significantly increased rapidly within 3 hours and progressively increase through 24 hours in both the peritoneal fluid and serum harvested postoperatively (N=6, p ≤ 0.05, one-way ANOVA with posthoc Bonferoni analysis).
