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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Protein Expr Purif. 2012 Oct 2;86(2):83–88. doi: 10.1016/j.pep.2012.09.012

Figure 3.

Figure 3

Far-UV CD spectra of purified WT CaM and methylated CaM in the presence and absence of calcium. CD spectra for WT CaM (left) and methylated CaM (right), in 5 mM HEPES buffer pH 7.5, were recorded at 25 °C in the presence (+ 1 mM CaCl2, more negative spectra, filled squares) and absence (+ 1 mM EGTA, less negative spectra, open circles) of calcium. Additional CD measurements in excess CaCl2 and EGTA show no significant differences.