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Caspase 3/7 activation by endogenously expressed RTA and RTA mutants in MAC-T cells. Caspase 3/7 activity was measured 19 h after transfection using the fluorescent SensoLyte Homogeneous AMC Caspase 3/7 Assay kit (AnaSpec). Bars represent mean ± S.E. of at least six experiments. White bars = preRTA constructs; black bars = mature RTA constructs. Significance compared to pre or mature WT RTA is indicated as: *, P < 0.001; #, P < 0.01; ‡, P < 0.05.
