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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Exp Hematol. 2012 Aug 8;40(12):1016–1027. doi: 10.1016/j.exphem.2012.08.001

Figure 1. CALM-AF10 AMLs acquire Flt3 mutations.

Figure 1

(A) and (B) Sequence tracings from CA10 AML samples 7468 and 7196 harboring 39 bp and 12 bp insertions in Flt3 exon 19. Red sequences represent the inserted sequences and the black sequences represent the WT sequences. (C) PCR analysis of genomic DNA from WT and CA10 infiltrated spleens using primers that flanked the ITD. Note the absence of the WT allele in samples 7196 and 7468. (D) Sequence tracings from two CA10 mice with a Flt3-TKD mutation. Note the presence of two peaks (mutant and WT) for the TKD mutations (indicated with a red arrow). (E) RQ-PCR analysis of Flt3 expression in WT BM (n=4), CA10 clinically healthy BM (n=3), CA10 AML (n=5), NHD13 clinically healthy BM (n=4) and NHD13 AML (n=16). 18S ribosomal RNA was used as an internal control and samples were normalized to WT BM.