Table 1.
MTEC genotype | |||||
---|---|---|---|---|---|
Estimated cell frequency | HIF-1α WT tumor-positive | HIF-1α-KO tumor-negative | HIF-1α WT | HIF-1α-KO | P valuesb |
Number of cells injected, % (n) | |||||
500 | 78% (7 of 9) | 0% (0 of 8) | 0.0023 | ||
200 | 90% (9 of 10) | 10% (1 of 10) | 0.0011 | ||
100 | 77% (10 of 13) | 9% (1 of 11) | 0.0003 | ||
50 | 69% (11 of 16) | 6% (1 of 16) | 0.0006 | ||
25 | 30% (6 of 20) | 0% (0 of 20) | 0.0202 | ||
10 | 35% (8 of 23) | 0% (0 of 22) | 0.0038 | ||
Estimated TIC frequency by ELDA (95% CI) | 1 of 82 (1 of 57 to 1 of 117) | 1 of 2,915 (1 of 925 to 1 of 9,193) | 5.79e-21 |
aELDA, Extreme Limiting Dilution Analysis; HIF-1α, hypoxia-inducible factor 1α; KO, knockout; MTEC, mammary tumor epithelial cell; TIC, tumor-infiltrating cell; WT, wild type. Frequency of tumors following limiting dilution transplantation of WT or KO MTECs is shown. The number of tumor-positive recipients per the total number of recipients at day 36 posttransplant and the P-values between genotypes for each cell density tested are listed. The estimated frequency of TICs was determined using the publicly available ELDA software. bP values were calculated using Fisher's exact test.