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. Author manuscript; available in PMC: 2013 Nov 10.
Published in final edited form as: Cell. 2012 Nov 9;151(4):847–858. doi: 10.1016/j.cell.2012.10.010

Figure 1. Altered Shh-dependent patterning in bby mutant embryos.

Figure 1

(A and B) Whole wild-type (WT) (A) and bby (B) mutant embryos at E10.5. (C-J) Neural patterning in WT and bby embryos at E10.5. Sections were taken at the forelimb level; the same pattern of expression of neural markers was seen at lumbar levels. Shh (C, D), Nkx2.2 (E, F) and Isl1 (G, H) are not expressed in the neural tube of bby mutant embryos. Pax6 (I, J) is expanded ventrally in bby mutants. Dorsal is up. Nuclei are stained with DAPI (blue). Scale bar =100 μm. (K-R) Limb patterning in WT and bby mutant embryos. Ptch1 (K, L) is not expressed in limb buds of bby embryos. Fgf4 (M, N) is expanded anteriorly in E10.5 bby mutants; expression of Hoxd11 (O, P) and Hand2 (Q, R) are expanded anteriorly in E11.5 bby mutants. Anterior is up. Please see also Figure S1 for information on the bby mutation.