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. 2012 Sep;87(8):485–493. doi: 10.3109/10520295.2012.687117

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© 2012 The Biological Stain Commission

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited.

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Fig. 3. — Low (a, c) and high (b, d) magnification micrographs of immunogold-labeled T. forsythensis cells after antigen retrieval by heating with the microwave oven. Microwaved at 100˚ C with 1% citraconic anhydride. Moderately decreased cytoplasm (a, c) compared to control cytoplasm (Fig. 1) without damage to the ultrastructural integrity of cytoplasmic membrane and outer membrane (b, d). Immunogold-particles in (b) and (d) showed greater reactivity than the control (Fig. 1). CM, cytoplasmic membrane; OM, outer membrane; S, S-layer.