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. 2012 Sep;87(8):485–493. doi: 10.3109/10520295.2012.687117

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© 2012 The Biological Stain Commission

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the source is credited.

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Fig. 5. — ESI using three-window method and parallel-EELS for T. forsythensis cells incubated in DAB medium with H₂O₂ and DAB. Nitrogen distribution corresponded to the outermost cell surface, the S-layer. a) Zero-loss transmission image. b) Background removed net ΔE_max 410 eV. c) Parallel-EELS analysis of the circled area in Fig. 5b. d) The second window of ΔE_w2 350 eV t. e) The first window of ΔE_w1 382 eV of nitrogen. f) ΔE_max 410 eV of K edge of nitrogen. g) Superimposed image of nitrogen distribution over the zero-loss image. CM, cytoplasmic membrane; OM, outer membrane; S, S-layer.