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. 2012 Jul 2;189(3):1265–1273. doi: 10.4049/jimmunol.1200761

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Copyright © 2012 by The American Association of Immunologists, Inc.

This article is distributed under The American Association of Immunologists, Inc., Reuse Terms and Conditions for Author Choice articles.

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FIGURE 4. — LT-K63 enhanced the TNF-α levels in cell culture supernatants after in vitro TT stimulation of spleen cells from mice immunized with Pnc1-TT as neonates. Spleen cells isolated 3 wk after priming of neonatal mice with Pnc1-TT (open column) or Pnc1-TT+LT-K63 (filled column) were stimulated with or without TT (10 μg/ml) for 48 h (20). TNF-α in the supernatants was measured by ELISA. The results are shown as pg TNF-α/ml (mean ± SD) in TT-stimulated cultures after subtracting TNF-α levels in unstimulated cultures. Student t test was applied, and p < 0.05 was considered statistically significant. Results shown are from two experiments for neonatal mice (n = 14 per group), with seven mice per group in each experiment.