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. 2012 Jul 27;189(5):2625–2634. doi: 10.4049/jimmunol.1102965

FIGURE 3.

FIGURE 3.

Specificity of the goat anti-gBK antiserum and purified anti-gBK Ab. Fixed-and-permeabilized U251 and normal human brain neurosphere cells were stained with either the preimmunized or postimmunized goat sera or with the purified goat anti-gBK Ab. Results in (A) show that (preimmunized) serum has some elevated background staining, whereas the immunized antiserum had enhanced staining. When the isotypic control and purified anti-gBK Abs were used, a lowered left shift was observed in both groups, as compared with the samples stained using whole sera. Results in (B) show the normal brain neurosphere cells to have minimal expression of gBK staining using the purified Ab, but they are positive for BK channels (C). Results in (D) show the expression of gBK by HEK cells that were either unmodified or transfected with the pcDNA/gBK plasmid for 48 h using the purified anti-gBK Ab.