Table I.
Primers used in this study.
| Primer names | Forward primers’ sequence (5′-3′) | Reverse primers’ sequence (5′-3′) | Product length (bp) | Usage |
|---|---|---|---|---|
| B19_Q-PCR_F / B19_Q-PCR_R | TGCAGATGCCCTCCACCCA | GCTGCTTTCACTGAGTTCTTC | 103 | Q-PCR |
| PVB-1f-1747 / PVB-1r-1967 | CACTATGAAAACTGGGCAATAAAC | AATGATTCTCCTGAACTGGTCC | 242 | Confirmation for Q-PCR, 1st round |
| PVB-2f-1765 / PVB-2r-1965 | ATAAACTACACTTTTGATTTCCCTG | TCTCCTGAACTGGTCCCG | 218 | Confirmation for Q-PCR, 2nd round |
| b19_f_701 / b19_r_4978 | AGACACTTCTGACTGGGAACCACTAAC | AGGGCACGGTGGGGAGTGTTTA | 4,287 | Nest PCR for genome analysis/PCR 1st round |
| b19_fN_725 / b19_r_2440 | TAACTCATACTAACAGACTAATGGCAAT | ACAAAGTCCCAAGCCTCCCC | 1,736 | Nest PCR for genome analysis/PCR 2nd round / sequencing |
| b19_f_2262 / b19_r_3505 | TCAGGAGAATCATTTGTCGGAAGC | AACCTTTGCCTCYTTTCCACT | 1,264 | Nest PCR for genome analysis/PCR 2nd round / sequencing |
| b19_f_3432 / b19_rN_4883 | GTTTTTAATTCCTAATGACCCAGAGC | TGTGGTGTTGTTTTGCATCTGTAG | 1,475 | Nest PCR for genome analysis/PCR 2nd round / sequencing |
| B19_f_1453 | ACTTTGAGCAGGTTATGTG | sequencing | ||
| B19_f_2966 | TACACAAGCCTGGGCAAGT | sequencing |