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. 2008 Nov 19;28(47):12581–12590. doi: 10.1523/JNEUROSCI.3338-08.2008

Figure 7.

Figure 7.

SiRNA-mediated knockdown of axonal COXIV levels decreases axonal respiration and ATP levels and diminishes NE uptake in distal axons. Two independent siRNA oligonucleotides (25 nm) targeted against COXIV mRNA were introduced in distal axons of SCG neurons by lipofection and COXIV mRNA (A) and protein levels (B) quantitated 24 h later by qRT-PCR and immunoblot analyses, respectively. Knockdown of axonal COXIV expression reduced axonal oxygen consumption (C), ATP levels (D), and [3H]NE uptake (E). Values are expressed as mean ± SEM and statistical significance evaluated by one-way ANOVA. *p < 0.03.