Table 1.
List of nuclear-encoded mitochondrial target genes of miR-338
| Target genes | Name | Energy (kcal/mol) | p value (×10−02) | RefSeq (transcript ID) |
|---|---|---|---|---|
| Mecr | Trans-2-enoyl-CoA reductase | −25.34 | 0.00006 | NM_017209 |
| Atp5g1* | ATP synthase lipid-binding protein | −24.82 | 1.296 | NM_017311 |
| Hspd1* | 60 kDa heat shock protein (Hsp60) | −23.86 | 0.979 | NM_022229 |
| Mrps18a | mitochondrial ribosomal protein S18A | −23.12 | 0.207 | NM_198756 |
| Sars2 | Seryl-tRNA synthetase, mitochondrial precursor | −21.96 | 0.325 | NM_001008859 |
| Sardh | Sarcosine dehydrogenase(SarDH) | −21.68 | 0.237 | NM_053664 |
| Fdxr | NADPH:adrenodoxin oxidoreductase (ferredoxin reductase) | −21.6 | 0.011 | NM_024153 |
| Clybl+ | Citrate lyase β subunit-like protein | −21.28 | 4.057 | XM_240311 |
| Dlst* | Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase | −20.94 | 0.015 | NM_001006981 |
| Hspa9a_predicted | Heat shock 70 kDa protein 9 | −20.1 | 0.054 | NM_001100658 |
| Iars2 | Isoleucine-tRNA synthetase 2, mitochondrial | −18.28 | 4.446 | XM_344185.3 |
| Ucp1 | Mitochondrial brown fat uncoupling protein 1 (UCP 1) | −16.92 | 0.724 | NM_012682 |
| Slc25a20 | Carnitine/acylcarnitine translocase | −16.81 | 0.293 | NM_053965 |
| Mrps10 | Mitochondrial 28S ribosomal protein S10 | −16.42 | 4.704 | NM_001008859 |
| Cox4i1* | Cytochrome c oxidase polypeptide IV | −14.87 | 0.332 | NM_017202 |
| Timm10+ | Mitochondrial import inner membrane translocase subunit Tim10. | −14.67 | 2.402 | NM_172074 |
| Top1mt+ | Mitochondrial topoisomerase I | −14.48 | 0.247 | NM_001002798 |
| Cyp11a1+ | Cytochrome P450 11A1, mitochondrial precursor | −12.33 | 2.168 | NM_017286 |
Computational prediction analysis (miRBase) identified 1009 putative miR-338 target genes, of which 18 were nuclear-encoded mitochondrial genes (miRBase, http://microrna.sanger.ac.uk).
*Gene products detected in both distal axons and cell soma by RT-qPCR. +Gene products detected in cell soma only by RT-qPCR.