Figure 5.

The binding of C1 to radiolabeled oligonucleotides containing either the wild-type (WT) sequence or mutations 2 (Mut2) or 3 (Mut3). Each labeled probe was used in three binding reactions, with probe alone and with 1 and 3 μg of C1 Myb protein, as indicated above each lane. The diagram below shows the wild-type oligonucleotide sequence, with the location of the two mutations boxed. Figure 2 contains the sequence changes in the mutant derivatives. The levels of activation of these mutations by C1 and B in the context of the −112-bp a2 promoter is shown below.