Figure 6.

a2-promoter sequences sufficient for C1 and B activation of a heterologous promoter. A synthetic promoter composed of a dimer of an oligonucleotide containing a2 sequences from −121 to −81 in front of a truncated, −59-bp CaMV 35S promoter was tested in the maize transient transformation system. The induction in the presence of C1 and B is shown. The boxed region is the C1-binding site that overlaps the crucial region identified by mutation 2. The lines represent the two regions that when mutated in the context of the −112 region had dramatic effects on C1- and B-mediated activation.