Subcellular distribution of Homer1c and its interacting partners, mGluR1α and mGluR5, in CA1. (A) Panels show immunohistochemistry of wildtype and H1-KO animals injected with either high dose H1c, low dose H1c or GFP. LM-WT mice express Homer1c at a lower level than CB57/BL6, as previously observed by Western blot analysis (Figure 1E). Labeling with GFAP reveals activated astrocytes in the stratum radiatum of KO+H1c animals, occurring both at the high and (to a lesser extent) the low dose of H1c. (B) Immunofluorescence images illustrating co-localization of mGluR5 (green, left column) and GFAP (red, middle column) in the hippocampus of the experimental animals shown. Double immunofluorescence is indicated in yellow in the merged image (right column). SP, stratum pyramidale; SO, stratum oriens-alveus; SR, stratum radiatum.