Fig. 4.

Effect of RUC-1, RUC-2, MSSM-1 and MSSM-2 on adhesion of platelets to immobilized fibrinogen at different concentrations. Washed platelets in buffer containing 1 mM Ca²⁺ and 1 mM Mg²⁺ were allowed to adhere to microtiter wells precoated with purified fibrinogen (50 µg/ml) in the absence or presence of the indicated compounds. After 1 h at RT the wells were washed and the adherent platelets detected by lysing them with 0.1 % Triton X-100 and assaying the released acid phosphatase activity