Fig. 7.

Priming effects of compounds. Tirofiban (0.5 µM), an RGDS peptide (100 µM), eptifibatide (1 µM), RUC-1 (100 µM), RUC-2 (1 µM), MSSM-1 (300 µM), or MSSM-2 (300 µM) were added to washed platelets and then the platelets were fixed with 1 % paraforrmaldeyde. After quenching the paraformaldehyde with glycine and washing, fluorescent fibrinogen (200 µg/ml) was added for 30 min at 37 °C and then, after washing again, bound fluorescent fibrinogen was detected by flow cytometry. The percentage of platelets with fluorescence values above 25 arbitrary units (AU) was recorded. The data shown is the percentage of platelets with values above 25 AU in the presence of each compound, minus the percentage in the untreated platelet sample. The latter averaged 4 ± 3 %