Figure 6. Effect of BQ123 on ERK activation in CHO cells stably transfected with ETA or ETB or both receptors.
The CHO cells expressing either ETA or ETB receptors were treated with 10 nM ET-1 with or without 1 mM BQ123 or 0.5 mM BQ788 for 5 minutes. After incubation the cell lysates were isolated and analyzed by western blot. GAPDH was used as a loading control.