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. Author manuscript; available in PMC: 2014 Feb 1.

Published in final edited form as: J Cell Physiol. 2013 Feb;228(2):322–329. doi: 10.1002/jcp.24132

The human phospho-antibody array detected phosphorylated proteins in untreated and treated cell lysates from donor control CONTROL-1 (A) and BMPR2 deletion HPAH-1 PASMC (B). The cells were either left untreated or treated with 10 nM ET-1 for 5 minutes with or without 1 uM BQ123. Profiles were created by quantifying the mean spot pixel densities normalized by positive control dots. Array signals from scanned X-ray film images were analyzed using image analysis software NIH ImageJ. The effect of ET-1 or ET1 + BQ123 was presented as fold change compared with the basal level of untreated sample. Only the targets with the fold change greater than 1.5 or less than 0.67 upon ET-1 stimulation were presented. The statistical analysis was performed using t-test. * p < 0.05 compared with untreated basal.

The human phospho-antibody array detected phosphorylated proteins in untreated and treated cell lysates from donor control CONTROL-1 (A) and BMPR2 deletion HPAH-1 PASMC (B). The cells were either left untreated or treated with 10 nM ET-1 for 5 minutes with or without 1 uM BQ123. Profiles were created by quantifying the mean spot pixel densities normalized by positive control dots. Array signals from scanned X-ray film images were analyzed using image analysis software NIH ImageJ. The effect of ET-1 or ET1 + BQ123 was presented as fold change compared with the basal level of untreated sample. Only the targets with the fold change greater than 1.5 or less than 0.67 upon ET-1 stimulation were presented. The statistical analysis was performed using t-test. * p < 0.05 compared with untreated basal.