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. Author manuscript; available in PMC: 2013 Jun 10.
Published in final edited form as: Exp Cell Res. 2012 Apr 4;318(10):1125–1133. doi: 10.1016/j.yexcr.2012.03.029

Figure 5. Effects of ET-1/ETB downstream inhibitors on the rate of DNA synthesis and cell division.

Figure 5

(A) [3H]-thymidine incorporation was performed as previously except some cells were pre-incubated 30 minutes with 100 uM DDA (adenylate cyclase inhibitor), 100 uM L-NAME (NOS inhibitior), 20 uM SP600125 (JNK inhibitor), 10 uM U0126 (MEK1/2 inhibitor) or 50 uM LY294002 (PI3K inhibitor). Cells were pulsed with [3H]-thymidine during the time interval of 3-5 hours after 10 nM ET-1 addition. Bar height indicates the CPM average and the error bars are the standard deviation. *p< 0.05 vs. untreated control. (B) The effect of LY294002 (PI3K inhibitor) on cell division in CHO ETB treated with 10 nM ET-1 as measured by real-time cell impedance. LY294002 (50 uM) was added 24 hours after the cells were seeded. ET-1 (10 nM) was added an hour later. To offset any small differences in cell growth before ET-1 addition, cell index values were normalized in the graph after ET-1 addition. All points in the curves represent the normalized cell index average and the error bars are the standard deviation.