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. Author manuscript; available in PMC: 2013 Oct 12.
Published in final edited form as: Mol Cell. 2012 Sep 20;48(1):16–27. doi: 10.1016/j.molcel.2012.08.016

Figure 6. The UPR compensates protein biogenesis defect during lipid imbalance.

Figure 6

Cells were grown to early log phase at 23°C and shifted to 37°C for 30 min (sec63-1) or 2 h in selective synthetic complete media in the absence (A) or presence (B) of 1 mM choline before being pulse-labeled at 37°C with L-[35S]-methionine/cysteine for 5 min followed by a chase at the indicated times. Immunoprecipitated proteins using anti-CPY or anti-Gas1p were resolved by SDS-PAGE and visualized by phosphoimager analysis.