Table 3.

Avoiding hairpin formation in 20 bases long sequences

No.	ΔG	Tanaka et al.	ΔG	Feldkamp	ΔG	EGNAS
	$\left[\frac{\mathit{kcal}}{\mathit{mol}}\right]$	5’ → 3’	$\left[\frac{\mathit{kcal}}{\mathit{mol}}\right]$	5’ → 3’	$\left[\frac{\mathit{kcal}}{\mathit{mol}}\right]$	5’ → 3’
1	1.1	ATGTACGTGAGATGCAGCAG	1.0	TAGTCGCGTGATTTGGAAGG	*	GAGAAGGAACACGATACAGC
2	1.1	ATCACTACTCGCTCGTCACT	0.9	GCTGTCTTTCGTCAATACCG	*	CCTTACACATTTCTTCCGCC
3	0.6	AGATGATCAGCAGCGACACT	0.3	CTGAACGGAATCTAGTAGCG	*	CACAATCAACTCTACCGCTC
4	0.5	TCTGTACTGCTGACTCGAGT	-0.1	GTCTACGGTTCTCTTACGCT	2.4	CCTGTCCTATCTTTCGCTTC
5	0.3	ACATCGACACTACTACGCAC	-0.2	AAAGCCGTCGTTTAAGGAGC	2.1	CTGGCTATGGAAACTGAACG
6	-0.2	GCTGACATAGAGTGCGATAC	-0.2	AATCGCAGTACAGATGGTGG	1.8	CTCGGTCTAAATCTGCTCTC
7	-0.5	TGTGCTCGTCTCTGCATACT	-0.2	GGATGACCAGAGCACTTCAA	1.8	GCCGTTATCCTCTGTTTGTC
8	-0.5	TCAGAGATACTCACGTCACG	-0.6	TACGTCTCGAACTGATAGCC	1.8	GGTTTATTGAGGTTGCGAGG
9	-0.8	CGAGTAGTCACACGATGAGA	-0.8	TGATCTTGTAAAGGCCAGGC	1.8	CCTCCGTATTTGCCTTGTTG
10	-0.9	CGAGACATCGTGCATATCGT	-0.8	TACGATACTTGGCGAGCCAT	1.5	GTTGTAGTTCGTTGGTGGTC
11	-1.2	AGACGAGTCGTACAGTACAG	-0.9	TGCAGAAAAACTATGCCGCC	1.5	CTTCGGCTGGTTCTATTCTG
12	-1.8	TATAGCACGAGTGCGCGTAT	-1.1	GCGCGGACAATTCATTGGTT	1.4	GGCTCACTCATCACACTAAC
13	-2.2	GATCTACGATCATGAGAGCG	-1.7	CCGCAATCCGGTGAAATTAG	1.4	CCTTTACGCCTGACTTTGAC
14	-2.3	GACAGAGCTATCAGCTACTG	-3.0	CTTAGGCAGGTGCCACATAT	1.3	CCTGCGTCTTATGTCTCTTG
15					0.9	GCGTGAATGAAGTGGAGTAG
16					0.9	GGATTACTTGCTTGGACTGG
						GC ends
				#		§
Lc		9		5		5
Lhp		5		5		2
Lsc		10		6		4
Lsl		7		5		5

Comparison with Tanaka et al. [17]Feldkamp et al. [20]. Comparison of 20 bases long sequences generated by Tanaka et al. [17], Feldkamp et al. [20] and EGNAS. Molar free enthalpies of the most stable hairpins.

^*No hairpin structure possible.

^#Without {GGG;CCC}.

^§Without {GGG;CCC;AAAA;TTTT}.