Table 7.

Combination of TAGs with Primers — comparison of TAGs

	TAGs
	Fanet al.	EGNAS1*
Average ΔG	-0.3 ± 0.9	1.6 ± 0.5
Minimum ΔG	-2.7	0.4
GC content	45% - 50%	50%
Lc	11	8
Lhp	4	2
Lsc	6	4
Lsl	7	8
Lni	7	3

Comparison of TAGs used by Fan et al. [8] and those generated by EGNAS. Intra- and interstrand properties. Molar free enthalpies (ΔG, kcal/mol) of the most stable hairpins. Averages ± standard deviations from 148 sequences.

^*GC ends and without {GGG;CCC;AAAAA;TTTTT}.