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. 2012 Nov 15;23(22):4335–4346. doi: 10.1091/mbc.E12-05-0420

FIGURE 3:

FIGURE 3:

Identification of potential folding helpers of IMS-DHFR. (A) Workflow of NiNTA pulldown of His-tagged DHFR constructs and subsequent quantitative analysis by label-free MS. (B) Isolated mitochondria were solubilized with digitonin-containing buffer in the absence of nucleotides or in the presence of ATP or ADP. Samples were incubated with NiNTA-Agarose beads and specifically bound proteins were eluted with Laemmli buffer containing 500 mM imidazole. Total (10%) and bound (100%) fractions were analyzed by SDS–PAGE and immunodecoration with the indicated antibodies.