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. 2012 Nov 15;23(22):4347–4361. doi: 10.1091/mbc.E12-04-0292

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© 2012 Lee et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — MITD1 is involved in cytokinesis. (A) HeLa cells were transfected with control siRNA (siCTL) or three different siRNAs against MITD1 (siMITD1). Cells were lysed and immunoblotted for MITD1. Actin levels were monitored to control for protein loading. (B) After the indicated siRNA transfections, cells were fixed and immunostained for CEP55 (green) and MITD1 (red). Merged images with DAPI staining are to the right. Insets are enlarged images of midbody regions. (C) HeLa cells transfected with the indicated siRNAs were stained with β-tubulin antibodies (green) and DAPI (blue). (D) Cells interconnected by midbodies and multinucleated cells were counted based on DAPI and β-tubulin staining as shown in C. Five hundred cells per group were analyzed in three independent experiments and graphed (means ± SEM). *p < 0.05; **p < 0.01. Bars, 10 μm (B, C), 2 μm (inset in B).