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. 2012 Nov 15;23(22):4362–4372. doi: 10.1091/mbc.E12-04-0293

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© 2012 Donovan and Corbo. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Morphometric analysis of INL cells. (A, B) SYTOX green counterstained sections were used to hand trace the nuclear outlines of cells in the INL. (B′) Boxed area in B shows large and irregularly shaped nuclei in the INL of RbCKO retina. (C, D) Representative digitized images from traced SYTOX Green–labeled nuclei were used to calculate the cross-sectional nuclear area of INL cells (unshaded) for control (Six3-Cre⁺;Rb1^lox/+; C) and RbCKO (D) retinas at P26. (E) Frequency histogram showing the distribution of cross-sectional nuclear area for cells in the INL of control (black) and RbCKO retinas (red). Cells from RbCKO show a distribution that extends toward much larger areas (inset). Scale bars, 20 μm.