Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Nov 15;23(22):4373–4382. doi: 10.1091/mbc.E12-04-0281

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 Cui et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell BD; are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 4: — Ndi1 is cleaved in mitochondria during apoptosis. (A) Ndi1 is cleaved when treated with Mn. NDI1-TAP yeast was treated or not treated with 8 mM Mn for 12 h, and the extracts were immunoblotted and probed with a TAP antibody. (B) Ndi1 cleavage occurs in the mitochondria. Both the full-length mature form of Ndi1 and the truncated Ndi1 were observed in the mitochondrial fraction, whereas only the truncated Ndi1 was found in the postmitochondrial fraction. Pgk1 served as the cytoplasm loading control, CoxIV as the mitochondrial loading control, and Idh1 as the matrix protein control. (C, D) The cleavage patterns of Ndi1 vs. treatment time and Mn concentration. (E, F) The survival rates of stressed NDI1-TAP yeast in C and D. (G, H) Ybh3 does not affect the apoptotic activity or release of Ndi1. (G) The proapoptotic activity of Ndi1 in the ybh3-deletion mutant tested by spotting assay. (H) The release of Ndi1 in NDI1-TAP,ybh3Δ background. Representative blots from at least three independent experiments are presented.